On March 25, 2015Maarten Rotman, Mayo clinic, Jacksonville wrote:
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We have used mouse anti Amyloid Beta 17-24 (4G8 from BioLegend, formerly Covance, prod. # SIG-39220) for Immuno Fluorescence staining on 5 µm fresh-frozen, both 4% PFA and acetone fixed human and mouse brain material at dilutions from 1:500 to 1:1000 in 4% milk PBS (mPBS). Secondary was usually Alexa Fluor 594 – goat anti mouse at 1:500 dilution (Life Technologies prod. # A-11032), sometimes Alexa Fluor 488 – goat anti mouse (Life Technologies prod. # A-11001). For immuno’s against amyloid beta deposits it works like a charm. For example pictures and protocol see doi:10.1016/j.nucmedbio.2015.03.003
On March 25, 2015 Maarten Rotman, Mayo clinic, Jacksonville wrote:
We have used mouse anti Amyloid Beta 17-24 (4G8 from BioLegend, formerly Covance, prod. # SIG-39220) for Immuno Fluorescence staining on 5 µm fresh-frozen, both 4% PFA and acetone fixed human and mouse brain material at dilutions from 1:500 to 1:1000 in 4% milk PBS (mPBS). Secondary was usually Alexa Fluor 594 – goat anti mouse at 1:500 dilution (Life Technologies prod. # A-11032), sometimes Alexa Fluor 488 – goat anti mouse (Life Technologies prod. # A-11001). For immuno’s against amyloid beta deposits it works like a charm. For example pictures and protocol see doi:10.1016/j.nucmedbio.2015.03.003