I have used this antibody for in situ hybridization in a dilution of 1:500. This antibody works so well for me.
For more information see Hao et al., The Lateral Hypothalamic and BNST GABAergic Projections to the Anterior Ventrolateral Periaqueductal Gray Regulate Feeding, Cell Reports, vol 28, issue 3, p616-624, July 16, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30824-1#%20).
Western blot
Not Rated
Immunostaining
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On November 2, 2017Julie Torvund-Jensen wrote:
5
I have used this antibody to detect GFP in transiently transfected HepG2 cells.
It gives a very nice and specific signal. Dilution 1:2000.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On July 13, 2016Signe Petersen, Aarhus University wrote:
4
Anti-GFP antibody (ab6556) ABCAM
I used this antibody for western blotting in a dilution of 1:2000 with great results. I blotted cell lysates from primary hippocampal neurons. The signal was not very powerful, however. No unspecific bands were detected.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On March 2, 2016Signe Petersen wrote:
5
I used this antibody on GFP-transfected, cultured mouse hippocampal neuron lysates at a dilution of 1:2000.
I conducted a western blot with excellent results.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On October 20, 2015Simon Mølgaard wrote:
5
This antibody works excellent for detecting GFP by western blot!
I used it on lysates from cultured mouse hippocampal neurons that had been transfected with GFP.
I used it at a dilution of 1:2000
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On November 12, 2014Sune Skeldal, Aarhus University wrote:
5
I have used this rabbit polyclonal antibody for western blotting with great success. It is sensitive not only to YFP but (according to the company) also other variants such as GFP and CFP. I use a dilution of 1:2000.
On November 19, 2019 Hao Wang, Zhejiang University School of Medicine wrote:
I have used this antibody for in situ hybridization in a dilution of 1:500. This antibody works so well for me.
For more information see Hao et al., The Lateral Hypothalamic and BNST GABAergic Projections to the Anterior Ventrolateral Periaqueductal Gray Regulate Feeding, Cell Reports, vol 28, issue 3, p616-624, July 16, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30824-1#%20).
On November 2, 2017 Julie Torvund-Jensen wrote:
I have used this antibody to detect GFP in transiently transfected HepG2 cells.
It gives a very nice and specific signal. Dilution 1:2000.
On July 13, 2016 Signe Petersen, Aarhus University wrote:
Anti-GFP antibody (ab6556) ABCAM
I used this antibody for western blotting in a dilution of 1:2000 with great results. I blotted cell lysates from primary hippocampal neurons. The signal was not very powerful, however. No unspecific bands were detected.
On March 2, 2016 Signe Petersen wrote:
I used this antibody on GFP-transfected, cultured mouse hippocampal neuron lysates at a dilution of 1:2000.
I conducted a western blot with excellent results.
On October 20, 2015 Simon Mølgaard wrote:
This antibody works excellent for detecting GFP by western blot!
I used it on lysates from cultured mouse hippocampal neurons that had been transfected with GFP.
I used it at a dilution of 1:2000
On November 12, 2014 Sune Skeldal, Aarhus University wrote:
I have used this rabbit polyclonal antibody for western blotting with great success. It is sensitive not only to YFP but (according to the company) also other variants such as GFP and CFP. I use a dilution of 1:2000.