Antibody works very nicely for ICC. We use rat primary neuronal cultures, PFA fixation and antibody incubation over night at 4°C. 1:500 dilution of recommended reconstitution.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On June 9, 2015Julia Abele, University of Magdeburg wrote:
4.5
PSD 95 from Synaptic Systems (mouse monoclonal, Cat. No. 124011) works reliably for western blotting. 1:1000 dilution, one dilution can be used up to three times.
This antibody is also suited for immunostainings 1:250.
On June 3, 2019 Julia Bär wrote:
Antibody works very nicely for ICC. We use rat primary neuronal cultures, PFA fixation and antibody incubation over night at 4°C. 1:500 dilution of recommended reconstitution.
On June 9, 2015 Julia Abele, University of Magdeburg wrote:
PSD 95 from Synaptic Systems (mouse monoclonal, Cat. No. 124011) works reliably for western blotting. 1:1000 dilution, one dilution can be used up to three times.
This antibody is also suited for immunostainings 1:250.
On November 11, 2014 Julia Abele, Magdeburg wrote:
specific in immunostainings