On December 22, 2014Camilla Gustafsen, Aarhus University wrote:
3
anti-LpL, mouse mAb clone 4G33. I have used this antibody for IP of LpL from primary mouse astrocytes (1µg/IP)
Western blot
Not Rated
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On September 28, 2014Simon Glerup, Mayo Clinic wrote:
5
This antibody is raised against lipoprotein lipase (LPL), which is known to be expressed by cortical astrocytes.
The staining seems specific, sensitive, and marks vesicular structures in cultures of mouse cortical glia.
Cells were permeabilized in PBS+0.1% TritonX100 and blocked in PBS+10% FBS.
Dilution for IF 2 ug/ml
On December 22, 2014 Camilla Gustafsen, Aarhus University wrote:
anti-LpL, mouse mAb clone 4G33. I have used this antibody for IP of LpL from primary mouse astrocytes (1µg/IP)
On September 28, 2014 Simon Glerup, Mayo Clinic wrote:
This antibody is raised against lipoprotein lipase (LPL), which is known to be expressed by cortical astrocytes.
The staining seems specific, sensitive, and marks vesicular structures in cultures of mouse cortical glia.
Cells were permeabilized in PBS+0.1% TritonX100 and blocked in PBS+10% FBS.
Dilution for IF 2 ug/ml