F4/80, FITC-conjugated chicken pAb, ImmuQuest IQ605FITC

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F480 FITC Iba1 4
Mouse cortical glia stained with anti-F4/80 (green), anti-Iba1 (Wako 019-19741, red), and Hoechst (blue)
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See list of additional F4/80 antibodies at Linscott’s Directory

One User Review

  • F4/80 is known as a marker for macrophages and microglia.

    We used this antibody to stain primary mouse cortical glia and the signal was indeed enriched in cells also positive for Iba1 (see above) but to a much lesser extent in cells positive for GFAP.
    Cells were permeabilized in PBS+0.1% TritonX100 and blocked in PBS+10% FBS.
    Dilution for IF 2 ug/ml

    Western blotNot Rated
    Immunostaining44444
    ELISANot Rated
    Flow cytometryNot Rated

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Western blot
Immunostaining
ELISA
Flow cytometry