On September 19, 2014Tina Storm, Aarhus University wrote:
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Goat anti NKCC1 (N-16)(raised against NKCC1 peptide of human origin)gave a weak signal with heavy background (1% milk blocking) when used in immunoflurescent detection on whole mouse eye paraffin sections (perfusion fixed with 2 % PFA). Visualized with Alexa donkey anti goat secondary antibody (1:600).
On September 19, 2014 Tina Storm, Aarhus University wrote:
Goat anti NKCC1 (N-16)(raised against NKCC1 peptide of human origin)gave a weak signal with heavy background (1% milk blocking) when used in immunoflurescent detection on whole mouse eye paraffin sections (perfusion fixed with 2 % PFA). Visualized with Alexa donkey anti goat secondary antibody (1:600).