Rabbit anti-biotin antibody (Cell signaling cat. 5597)

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Fig 2 Kernohan
Kernohan et al. used this antibody in MethodsX 2014. The figure from the paper shows confocal images of dual labelled DNA FISH in fixed mouse brain sections. (a) Example of field of view of neonatal mouse brain. Each cell has two foci per probe marking the two alleles. (b) Example of a collapsed image showing nuclear DNA foci and (c) its corresponding three dimensional rendering conducted with the Volocity software package. This data can be used to measure relative distances, signal volume or number of foci within the cell.

See list of additional Biotin antibodies at Linscott’s Directory

One User Review

  • This antibody works ok for immunofluorescence, but does produce some background. With added washes you can obtain a specific signal. Used with immuno-DNA-fish.

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