Works excellent for detecting BDNF! A clear signal was seen with very little backgrounds
used 10ug/ml on cultured hippocampal neurons.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On March 20, 2014Lone Tjener Pallesen wrote:
1
We tested this antibody for WB diluted 1:750 and observed no bands using 50, 100 and 500 ng of BDNF and proBDNF (cleavage-resistant mutant).
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 10, 2012Simon Glerup, Aarhus wrote:
5
This BDNF antibody works great for immunostaining of hippocampal neurons (mice). Staining appears vesicular.
The BDNF antibody also works for detecting recombinant proBDNF binding to 293 cells transfected with p75NTR.
BDNF antibody concentration 10 ug/ml
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On May 20, 2012Simon G, Aarhus wrote:
4
This BDNF antibody is great for immunoprecipitation of endogenous BDNF secreted from primary mouse hippocampal neurons.
Prior to the IP, BDNF was metabolically labelled.
Western blot
Not Rated
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On January 7, 2012Anja Aagaard, Aarhus wrote:
1
This antibody was kindly provided by Yves Barde.
We tried on mouse hippocampal neurons 14 DIV fixed with formaldehyde. We didn´t see anything.
On May 8, 2015 Simon Mølgaard wrote:
Works excellent for detecting BDNF! A clear signal was seen with very little backgrounds
used 10ug/ml on cultured hippocampal neurons.
On March 20, 2014 Lone Tjener Pallesen wrote:
We tested this antibody for WB diluted 1:750 and observed no bands using 50, 100 and 500 ng of BDNF and proBDNF (cleavage-resistant mutant).
On November 10, 2012 Simon Glerup, Aarhus wrote:
This BDNF antibody works great for immunostaining of hippocampal neurons (mice). Staining appears vesicular.
The BDNF antibody also works for detecting recombinant proBDNF binding to 293 cells transfected with p75NTR.
BDNF antibody concentration 10 ug/ml
On May 20, 2012 Simon G, Aarhus wrote:
This BDNF antibody is great for immunoprecipitation of endogenous BDNF secreted from primary mouse hippocampal neurons.
Prior to the IP, BDNF was metabolically labelled.
On January 7, 2012 Anja Aagaard, Aarhus wrote:
This antibody was kindly provided by Yves Barde.
We tried on mouse hippocampal neurons 14 DIV fixed with formaldehyde. We didn´t see anything.
Concentration used: 10 ug/ml