On April 22, 2014Lone Tjener Pallesen, Aarhus University wrote:
3.5
WB: The antibody works well diluted 1:1000. For western blots, incubate membrane with diluted antibody overnight at 4°C in 5% w/v skimmed milk, 1X TBS, 0.1% Tween-20.
IF: Used 1:50 for detection of endogenous levels in HEK cells.
On April 22, 2014 Lone Tjener Pallesen, Aarhus University wrote:
WB: The antibody works well diluted 1:1000. For western blots, incubate membrane with diluted antibody overnight at 4°C in 5% w/v skimmed milk, 1X TBS, 0.1% Tween-20.
IF: Used 1:50 for detection of endogenous levels in HEK cells.