On April 3, 2014Piotr Siupka , Aarhus University wrote:
1
Dilution: 1/100
Tested on HEK293 stably transfected with RFP tagged protein, lots of background observed. When tested on non-transfected HEK293 plenty of punctate spots detected. So not a good antibody for immunostaining and confocal microscopy, it binds unspecifically.
On April 3, 2014 Piotr Siupka , Aarhus University wrote:
Dilution: 1/100
Tested on HEK293 stably transfected with RFP tagged protein, lots of background observed. When tested on non-transfected HEK293 plenty of punctate spots detected. So not a good antibody for immunostaining and confocal microscopy, it binds unspecifically.