On March 16, 2016Sune Skeldal, Aarhus University wrote:
3
Detects human calnexin in HEK293 cells in immunocytochemistry reasonable well using a dilution of 1:20. However a background control is obviously not available here.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On June 19, 2014Mads Fuglsang Kjolby, Mayo Clinic wrote:
4
HepG2 cells and/or primary hepatocytes were fixed in 4% PFA, permeabilized (0.1% Triton-X 100), blocked in 10% FCS in PBS, and incubated with anti-Calnexin 1:250 (ab22595, Abcam, Cambridge, England) for 24 hrs at 4°C.
In Kjolby et al. Cell Metab, Suppl material, Figure S3
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 2, 2013Simon Glerup, Aarhus University wrote:
4
This antibody seems to work OK for staining of calnexin i HepG2 cells.
Cells were fixed in 4% PFA for 20 min at RT.
Washing was carried out using PBS containing 0.1% Triton X100.
Dilution 5 ug/ml.
See image above. Calnexin (red). Cells are also stained for sortilin (green, mAb F11) and nuclei using Hoechst (blue).
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On May 30, 2013Camilla Gustafsen wrote:
4
This antibody can be used for WB of mouse liver homogenates. We used it as ER marker after subcellular fraktionation. No background bands. Use 1:1000 or dilute more
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On March 16, 2016 Sune Skeldal, Aarhus University wrote:
Detects human calnexin in HEK293 cells in immunocytochemistry reasonable well using a dilution of 1:20. However a background control is obviously not available here.
On June 19, 2014 Mads Fuglsang Kjolby, Mayo Clinic wrote:
HepG2 cells and/or primary hepatocytes were fixed in 4% PFA, permeabilized (0.1% Triton-X 100), blocked in 10% FCS in PBS, and incubated with anti-Calnexin 1:250 (ab22595, Abcam, Cambridge, England) for 24 hrs at 4°C.
In Kjolby et al. Cell Metab, Suppl material, Figure S3
On November 2, 2013 Simon Glerup, Aarhus University wrote:
This antibody seems to work OK for staining of calnexin i HepG2 cells.
Cells were fixed in 4% PFA for 20 min at RT.
Washing was carried out using PBS containing 0.1% Triton X100.
Dilution 5 ug/ml.
See image above. Calnexin (red). Cells are also stained for sortilin (green, mAb F11) and nuclei using Hoechst (blue).
On May 30, 2013 Camilla Gustafsen wrote:
This antibody can be used for WB of mouse liver homogenates. We used it as ER marker after subcellular fraktionation. No background bands. Use 1:1000 or dilute more