Calnexin rabbit anti-human Abcam ab22595

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4 User Reviews

  • Detects human calnexin in HEK293 cells in immunocytochemistry reasonable well using a dilution of 1:20. However a background control is obviously not available here.

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  • HepG2 cells and/or primary hepatocytes were fixed in 4% PFA, permeabilized (0.1% Triton-X 100), blocked in 10% FCS in PBS, and incubated with anti-Calnexin 1:250 (ab22595, Abcam, Cambridge, England) for 24 hrs at 4°C.
    In Kjolby et al. Cell Metab, Suppl material, Figure S3

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  • This antibody seems to work OK for staining of calnexin i HepG2 cells.
    Cells were fixed in 4% PFA for 20 min at RT.
    Washing was carried out using PBS containing 0.1% Triton X100.
    Dilution 5 ug/ml.
    See image above. Calnexin (red). Cells are also stained for sortilin (green, mAb F11) and nuclei using Hoechst (blue).

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  • This antibody can be used for WB of mouse liver homogenates. We used it as ER marker after subcellular fraktionation. No background bands. Use 1:1000 or dilute more

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