On August 23, 2019Camilla Gustafsen, Aarhus University, Denmark wrote:
0
This mAb was generated in the lab of Professor Claus Munck Petersen, Aarhus University. It is commercially available from Sigma Aldrich/Merck as MABN1793.
On December 15, 2014Laura Johnsen wrote:
5
I have used the SorLA antibody 20:C11 on cryosectioned, PFA fixated mouse dorsal root ganglia, on which it works fine.
I have used it in combination with Fab buffer (Donkey anti-mouse)
Dilution: 1:500
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On April 11, 2014Thaneas Prabakaran, Aarhus University wrote:
5
It works very well for immunostaining of PFA fixed cells at a dilution of 1:100
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On March 7, 2014Helene Andersen wrote:
5
I have used this antibody for immunostainings on adult mouse barin tissue. It worked very well for detection of SorLA.
concentration 10ug/ml
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On January 29, 2014Simon Mølgaard wrote:
5
I have used this antibody for immunostainings on adult mouse barin tissue as well as cultured hipoocampal neurons. In both cases this antibody worked very well for detection of SorLA.
concentration 10ug/ml
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On December 10, 2013Mathias Kaas Ollendorff wrote:
4
We used this SorLA mAb for ELISA on human serum. Development takes about 20-30 minutes, gives a nice std curve and good consistent measurements for serum diluted 5, 10 and 20 times in 1 % BSA.
For best results we used Nunc MaxiSorp 96 well plates.
I have used the 20C11 antibody for staining of brain sections from cortex (nice neuron staining in vesicle strucures / TGN) and also in dopaminergic neurons in substantia nigra in mesencephalon (see Glerup et al., Cell reports, 2013) (double staining with anti-TH and SorLA).
OBS: In mice it is necessary to block with fab fragments if the tissue has to much background.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On August 20, 2013Stine Klinger wrote:
0
Not reactive against rat SorLA
On December 21, 2011Simon Glerup, Aarhus University wrote:
4.5
This is a nice SorLA mAb produced in Claus M. Petersen lab, Aarhus. Cited in Klinger et al. Journal of Cell Science 2011
I have used it for Western blotting on brain from wt and SorLA knockouts. It detects a specific band around 250 kDa.
It is great for staining glia and neurons both in ICC and IHC. Knockouts are blank.
For WB 1 ug/ml
For ICC 10 ug/ml
The image above shows a primary mouse cortical astrocyte stained using SorLA mAb 20C11 (green) and GFAP (red, DAKO)
On August 23, 2019 Camilla Gustafsen, Aarhus University, Denmark wrote:
This mAb was generated in the lab of Professor Claus Munck Petersen, Aarhus University. It is commercially available from Sigma Aldrich/Merck as MABN1793.
On December 15, 2014 Laura Johnsen wrote:
I have used the SorLA antibody 20:C11 on cryosectioned, PFA fixated mouse dorsal root ganglia, on which it works fine.
I have used it in combination with Fab buffer (Donkey anti-mouse)
Dilution: 1:500
On April 11, 2014 Thaneas Prabakaran, Aarhus University wrote:
It works very well for immunostaining of PFA fixed cells at a dilution of 1:100
On March 7, 2014 Helene Andersen wrote:
I have used this antibody for immunostainings on adult mouse barin tissue. It worked very well for detection of SorLA.
concentration 10ug/ml
On January 29, 2014 Simon Mølgaard wrote:
I have used this antibody for immunostainings on adult mouse barin tissue as well as cultured hipoocampal neurons. In both cases this antibody worked very well for detection of SorLA.
concentration 10ug/ml
On December 10, 2013 Mathias Kaas Ollendorff wrote:
We used this SorLA mAb for ELISA on human serum. Development takes about 20-30 minutes, gives a nice std curve and good consistent measurements for serum diluted 5, 10 and 20 times in 1 % BSA.
For best results we used Nunc MaxiSorp 96 well plates.
Concentration was 1 ug/ml
On December 2, 2013 Mads Kjolby wrote:
I have used the 20C11 antibody for staining of brain sections from cortex (nice neuron staining in vesicle strucures / TGN) and also in dopaminergic neurons in substantia nigra in mesencephalon (see Glerup et al., Cell reports, 2013) (double staining with anti-TH and SorLA).
OBS: In mice it is necessary to block with fab fragments if the tissue has to much background.
On August 20, 2013 Stine Klinger wrote:
Not reactive against rat SorLA
On December 21, 2011 Simon Glerup, Aarhus University wrote:
This is a nice SorLA mAb produced in Claus M. Petersen lab, Aarhus. Cited in Klinger et al. Journal of Cell Science 2011
I have used it for Western blotting on brain from wt and SorLA knockouts. It detects a specific band around 250 kDa.
It is great for staining glia and neurons both in ICC and IHC. Knockouts are blank.
For WB 1 ug/ml
For ICC 10 ug/ml
The image above shows a primary mouse cortical astrocyte stained using SorLA mAb 20C11 (green) and GFAP (red, DAKO)