2 User Reviews

  • Used for Immunofluorescence of transfected RN22 cells treated with 4% PFA in PBS for 5 min. Washed 10 min in PBS. And penetrated with 0.5 % Triton x-100 in PBS for 7 min at 4 C.
    Best dilution was 1:40.
    Very strong signal. Try further dilutions for lesser signal, for Co-IF.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Used for Western blotting of RN22 cells in a dilution 1:1000, gives two Nestin bands at approximate 250 and 220 kDa.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex