On February 13, 2014Ann-Kathrin Reuschlein, Aarhus University wrote:
5
Used for Immunofluorescence of transfected RN22 cells treated with 4% PFA in PBS for 5 min. Washed 10 min in PBS. And penetrated with 0.5 % Triton x-100 in PBS for 7 min at 4 C.
Best dilution was 1:40.
Very strong signal. Try further dilutions for lesser signal, for Co-IF.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 13, 2014Ann-Kathrin Reuschlein, Aarhus University wrote:
5
Used for Western blotting of RN22 cells in a dilution 1:1000, gives two Nestin bands at approximate 250 and 220 kDa.
On February 13, 2014 Ann-Kathrin Reuschlein, Aarhus University wrote:
Used for Immunofluorescence of transfected RN22 cells treated with 4% PFA in PBS for 5 min. Washed 10 min in PBS. And penetrated with 0.5 % Triton x-100 in PBS for 7 min at 4 C.
Best dilution was 1:40.
Very strong signal. Try further dilutions for lesser signal, for Co-IF.
On February 13, 2014 Ann-Kathrin Reuschlein, Aarhus University wrote:
Used for Western blotting of RN22 cells in a dilution 1:1000, gives two Nestin bands at approximate 250 and 220 kDa.