On November 2, 2016Andrea E. Toth, Aarhus University wrote:
5
This antibody works excellently for immunofluorescence staining in mouse (b.End3), human (hCMEC/D3) brain endothelial cell line and in primary porcine brain endothelial cells. Dilution 1:200. Fixation was done with 4% paraformaldehyde and cells were permeabilized with 0.25% Triton X-100.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On March 4, 2014Camilla Gustafsen, Aarhus University wrote:
5
Very nice for IF using HepG2 cells. The antibody detects vesicular structures colocalizing with the retromer-associated receptor SorLA.
Cells were fixed in 4% PFA and permeabilized using 0.1% Triton X100.
Dilution 1:200
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On October 25, 2013Morten Nielsen, Aarhus University wrote:
5
Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells.
Very strong and specific vesicular staining. An excellent antibody for fluorescent imaging.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
The picture above shows SHSY5Y cells stained for Vps35 using this antibody (green). Nuclei are stained using Hoechst (blue).
On November 2, 2016 Andrea E. Toth, Aarhus University wrote:
This antibody works excellently for immunofluorescence staining in mouse (b.End3), human (hCMEC/D3) brain endothelial cell line and in primary porcine brain endothelial cells. Dilution 1:200. Fixation was done with 4% paraformaldehyde and cells were permeabilized with 0.25% Triton X-100.
On March 4, 2014 Camilla Gustafsen, Aarhus University wrote:
Very nice for IF using HepG2 cells. The antibody detects vesicular structures colocalizing with the retromer-associated receptor SorLA.
Cells were fixed in 4% PFA and permeabilized using 0.1% Triton X100.
Dilution 1:200
On October 25, 2013 Morten Nielsen, Aarhus University wrote:
Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells.
Very strong and specific vesicular staining. An excellent antibody for fluorescent imaging.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
The picture above shows SHSY5Y cells stained for Vps35 using this antibody (green). Nuclei are stained using Hoechst (blue).