On October 25, 2013Morten Nielsen, Aarhus University wrote:
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Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells.
Weak signal in cytoplasm and nucleus was observed, but AP2A1 should normally not be present in the nucleus. Nucleus background may be reduced with blocking. However, the antibody is still weak for fluorescent imaging.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
The picture above shows SHSY5Y cells stained for AP2A1 using this antibody (green). Nuclei are stained using Hoechst (blue).
On October 25, 2013 Morten Nielsen, Aarhus University wrote:
Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells.
Weak signal in cytoplasm and nucleus was observed, but AP2A1 should normally not be present in the nucleus. Nucleus background may be reduced with blocking. However, the antibody is still weak for fluorescent imaging.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
The picture above shows SHSY5Y cells stained for AP2A1 using this antibody (green). Nuclei are stained using Hoechst (blue).