This antibody did not work for me in both immunostaining and WB.
IF: Primary hippocampal neruons from mice were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a both 0,5ug/mL, 1ug/mL and 2ug/mL dilution in blocking buffer. No clear signal was observed using these concentrations.
WB: I used 1:5000 dilution of primary hippocampal neurons and was unable to detect HSPA8.
Western blot
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On October 25, 2013Morten Nielsen, Aarhus University wrote:
2
Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells.
Weak signal in cytoplasm and nucleus was observed, corresponding to expected localisation. However, not optimal for fluorescent imaging.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
On November 28, 2017 Anders Dalby wrote:
This antibody did not work for me in both immunostaining and WB.
IF: Primary hippocampal neruons from mice were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a both 0,5ug/mL, 1ug/mL and 2ug/mL dilution in blocking buffer. No clear signal was observed using these concentrations.
WB: I used 1:5000 dilution of primary hippocampal neurons and was unable to detect HSPA8.
On October 25, 2013 Morten Nielsen, Aarhus University wrote:
Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells.
Weak signal in cytoplasm and nucleus was observed, corresponding to expected localisation. However, not optimal for fluorescent imaging.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.