On October 25, 2013Morten Nielsen, Aarhus University wrote:
1
Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells. No positive signal was observed.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
On October 25, 2013 Morten Nielsen, Aarhus University wrote:
Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells. No positive signal was observed.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.