I have also tested this on cultured hippocampal neurons stimulated with 1 nM of BDNF for 10 minutes. This works great to detect phosphorylation of AKT.
Dilution 1:800
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 25, 2012Simon Jensen wrote:
4
This antibody was tested on 293 cells in culture. The cells were stimulated with either 0, 1, 10 or 100nM of insulin before being fixed and stained. This antibody showed increased staining af cells stimulated with 1 and 10nM, compared to unstimulated.
Dilution 1:800
On January 29, 2014 Simon Mølgaard wrote:
I have also tested this on cultured hippocampal neurons stimulated with 1 nM of BDNF for 10 minutes. This works great to detect phosphorylation of AKT.
Dilution 1:800
On November 25, 2012 Simon Jensen wrote:
This antibody was tested on 293 cells in culture. The cells were stimulated with either 0, 1, 10 or 100nM of insulin before being fixed and stained. This antibody showed increased staining af cells stimulated with 1 and 10nM, compared to unstimulated.
Dilution 1:800