I have also tested this antibody on cultured hippocampal neurons stimulated with 1 nM of BDNF for 10 minutes with very limited succes.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 25, 2012Simon Jensen wrote:
1
This antibody was tested on 293 cells in culture. The cells were stimulated with either 0, 1, 10 or 100nM of insulin before being fixed and stained. This antibody showed almost no difference between stimulated and unstimulated.
Dilution 1:100
On January 29, 2014 Simon Mølgaard wrote:
I have also tested this antibody on cultured hippocampal neurons stimulated with 1 nM of BDNF for 10 minutes with very limited succes.
On November 25, 2012 Simon Jensen wrote:
This antibody was tested on 293 cells in culture. The cells were stimulated with either 0, 1, 10 or 100nM of insulin before being fixed and stained. This antibody showed almost no difference between stimulated and unstimulated.
Dilution 1:100