This antibody works great in a home-made ELISA as capture antibody for detection of human SorCS1.
I have used 10 ug/ml when coating the maxisorp plate (55 mM sodium bicarbonate, pH 9.8).
Details can be acquired upon request.
Western blot
Not Rated
Immunostaining
Not Rated
IP
Not Rated
ELISA
Flow cytometry
Not Rated
Luminex
Not Rated
On November 22, 2012Jenny Blechingberg Friis, University of Aarhus wrote:
4
This antibody is an old antibody (homemade??) and stored in 4 degrees. It was used to stain CHO-cells stable expressing mouse SorCS1 variant c. The membrane of CHO/mSorCS1c stain weakly in a dotted pattern and the untransfected CHO-cells are without staining at 3 ug/ml.
On August 11, 2016 Melkam Kebede, University of Sydney wrote:
Where can one get this antibody? Really interested in trying it in our cells?
Thanks,
On October 8, 2015 Peter Lund Ovesen, University of Aarhus wrote:
This antibody (9,6 ug/ul) works excellent for detecting human and mouse SorCS1 in lysates of HeLa cells overexpressing the protein.
Western blot Dilution 1:2500
On December 2, 2013 Mads Kjolby, University of Aarhus wrote:
This antibody works great in a home-made ELISA as capture antibody for detection of human SorCS1.
I have used 10 ug/ml when coating the maxisorp plate (55 mM sodium bicarbonate, pH 9.8).
Details can be acquired upon request.
On November 22, 2012 Jenny Blechingberg Friis, University of Aarhus wrote:
This antibody is an old antibody (homemade??) and stored in 4 degrees. It was used to stain CHO-cells stable expressing mouse SorCS1 variant c. The membrane of CHO/mSorCS1c stain weakly in a dotted pattern and the untransfected CHO-cells are without staining at 3 ug/ml.