On November 15, 2015Camilla Gustafsen, Aarhus University wrote:
1
This antibody (1:1000) does not work for WB detection of endogenous LDLR in HepG2 cell lysate.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On March 25, 2015Laura Tvilling Poulsen (Aarhus University) wrote:
5
Anti-LDLR, ab60107, mouse.
Using this antibody (1:100) we saw an intense IF staining of endogenous LDLR in HepG2 cells. As control we used HepG2 cells with overexpression of PCSK9, and the antibody signal in these cells was, as expected, very weak.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On October 30, 2014Simon Glerup, Aarhus University wrote:
1
This antibody does not work for IP of endogenous LDL receptor from HEK293 cells.
We did IPs from metabolically labelled cells (S35)and saw a lot of unspecific bands. The antibody even pulled down the related receptor SorLA.
Western blot
Not Rated
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 22, 2012Camilla Gustafsen wrote:
5
This antibody works very well for IF staining of LDLR in HepG2 cells. Use 10 µg/ml.
On November 15, 2015 Camilla Gustafsen, Aarhus University wrote:
This antibody (1:1000) does not work for WB detection of endogenous LDLR in HepG2 cell lysate.
On March 25, 2015 Laura Tvilling Poulsen (Aarhus University) wrote:
Anti-LDLR, ab60107, mouse.
Using this antibody (1:100) we saw an intense IF staining of endogenous LDLR in HepG2 cells. As control we used HepG2 cells with overexpression of PCSK9, and the antibody signal in these cells was, as expected, very weak.
On October 30, 2014 Simon Glerup, Aarhus University wrote:
This antibody does not work for IP of endogenous LDL receptor from HEK293 cells.
We did IPs from metabolically labelled cells (S35)and saw a lot of unspecific bands. The antibody even pulled down the related receptor SorLA.
On November 22, 2012 Camilla Gustafsen wrote:
This antibody works very well for IF staining of LDLR in HepG2 cells. Use 10 µg/ml.