This antibody worked great for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.
Dilution used: 1:5000
For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).
Excellent antibody for staining cholera toxin subunit b bound and internalized by 293 cells and hipppcampal neurons.
Dilution 1:1000
Western blot
Not Rated
Immunostaining
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On September 12, 2012Simon Glerup wrote:
2
We used this cholera toxin B antibody at dilution 1:1000 for the detection retrogradely transported cholera toxin B subunit on cryosections of PFA-fixed mouse brain. The antibody was not very useful. There was strong cross reaction with blood vessels.
On October 17, 2019 David I. Hughes, University of Glasgow wrote:
This antibody worked great for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.
Dilution used: 1:5000
For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).
Hughes Laboratory:
https://specciebear.wixsite.com/spinal-cord-hughes
On November 24, 2012 Simon Glerup, Aarhus wrote:
Excellent antibody for staining cholera toxin subunit b bound and internalized by 293 cells and hipppcampal neurons.
Dilution 1:1000
On September 12, 2012 Simon Glerup wrote:
We used this cholera toxin B antibody at dilution 1:1000 for the detection retrogradely transported cholera toxin B subunit on cryosections of PFA-fixed mouse brain. The antibody was not very useful. There was strong cross reaction with blood vessels.