Works very nicely for IF (HEK293 and HepG2 cells).
I have used a 1:500 dilution and overnight incubation,
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On August 24, 2016Aaron wrote:
5
For IF, dilution at 1:500 to 1:1000 is working good after overnight incubation.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On March 15, 2016Sune Skeldal, Aarhus University wrote:
4
I used this antibody as a marker for TGN in HEK293 cells in confocal fluorescence with good results (1:100).
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On May 26, 2013Camilla Gustafsen wrote:
0
This antibody is very good for immunostaining of HepG2 cells. We used it 1:100, but I reckon that it can be diluted more and still give a nice result
On June 25, 2012Simon Glerup, Aarhus wrote:
4
We used this TGN46 antibody 1:100. It works fine for immunostaining of HEK293 cells.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On March 9, 2012Simon Glerup, Aarhus wrote:
3
We used it in Western blotting on sucrose gradient fractions.
It recognizes a very faint band of the correct size at the expected density. A stronger (but still weak) band is observed in the post nuclear supernatant of 293 cells.
On February 6, 2018 Julie Torvund-Jensen wrote:
Works very nicely for IF (HEK293 and HepG2 cells).
I have used a 1:500 dilution and overnight incubation,
On August 24, 2016 Aaron wrote:
For IF, dilution at 1:500 to 1:1000 is working good after overnight incubation.
On March 15, 2016 Sune Skeldal, Aarhus University wrote:
I used this antibody as a marker for TGN in HEK293 cells in confocal fluorescence with good results (1:100).
On May 26, 2013 Camilla Gustafsen wrote:
This antibody is very good for immunostaining of HepG2 cells. We used it 1:100, but I reckon that it can be diluted more and still give a nice result
On June 25, 2012 Simon Glerup, Aarhus wrote:
We used this TGN46 antibody 1:100. It works fine for immunostaining of HEK293 cells.
On March 9, 2012 Simon Glerup, Aarhus wrote:
We used it in Western blotting on sucrose gradient fractions.
It recognizes a very faint band of the correct size at the expected density. A stronger (but still weak) band is observed in the post nuclear supernatant of 293 cells.
Dilution 1:500