A rabbit antiserum raised against an intracellular epitope of the human nerve growth factor receptor p75 (Promega, Madison, WI, USA; G323A) was consistently useful to reveal this marker which is in forebrain tissues exclusively by cholinergic projection neurons. This is exemplified by immunofluorescence labelling of the basal forebrain in hamsters (Härtig et al 2007, Fig 4). Notably, this immunoreagent is appropriate for the detection of p75 in various mammalian species – in contrast to antibodies recognizing extracellular epitopes frequently only in rat or mice or human and rabbit.
On January 6, 2019 Wolfgang Härtig, University of Leipzig wrote:
I can highly recommend this antibody:
A rabbit antiserum raised against an intracellular epitope of the human nerve growth factor receptor p75 (Promega, Madison, WI, USA; G323A) was consistently useful to reveal this marker which is in forebrain tissues exclusively by cholinergic projection neurons. This is exemplified by immunofluorescence labelling of the basal forebrain in hamsters (Härtig et al 2007, Fig 4). Notably, this immunoreagent is appropriate for the detection of p75 in various mammalian species – in contrast to antibodies recognizing extracellular epitopes frequently only in rat or mice or human and rabbit.
Härtig et al. 2007: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1460-9568.2006.05250.x
On January 27, 2013 Anja A wrote:
This antibody works for immunostaining of p75 in transfected HEK293 cells.
Dilution 1:200