This antibody worked well for detecting LAMP1 in lysates from murine primary cortical neurons. Clear bands were detected after 30 sec. of exposure with ECl prime (Amersham).
This antibody works very well for the detection of LAMP1 in lysates of primary mouse neuronal cultures through WB. (Dilution of 1:1000)
It did not work for HEK293 cell lysates, probably because it does not cross-react with the human LAMP1.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On March 9, 2012Simon Glerup, Aarhus wrote:
1
This antibody stains vesicles in 293 cells that appear to be located in the nucleus or just above it.
I doubt that these are lysosomes! 293 cells were permeabilized with 0.1% TritonX100
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 29, 2012Anja A, Aarhus wrote:
0
This antibody also works for staining of 293 cells.
On February 15, 2012Simon Glerup, Aarhus wrote:
4
We use this antibody as a lysosomal marker in mouse-derived cell lines, e.g. primary astrocytes and hippocampal neurons.
Dilution 1:300.
NB. For human cell lines, we use mAb H4A3.
It is important to consider what detergent is used for permeabilization of cells. Saponin appears to give much better lysosome stainings than Triton X-100.
For inhibition of lysosomal degradation and better visualization of lysosomes, incubate with 50 ug/ml leupeptin and pepstatin for 24 h before fixing the cells. During this period, the leupeptin/pepstatin medium has to be changed every six hours.
On October 2, 2019 Anders Dalby wrote:
Works great for detection of LAMP-1 in primary murine neurons (cortical). A clear band with no background signal was obtained after short exposure.
Dilution 1:1000
On July 1, 2019 Trine Rasmussen wrote:
This antibody worked well for detecting LAMP1 in lysates from murine primary cortical neurons. Clear bands were detected after 30 sec. of exposure with ECl prime (Amersham).
Dilution 1:1000
On July 1, 2019 Sérgio Almeida wrote:
This antibody works very well for the detection of LAMP1 in lysates of primary mouse neuronal cultures through WB. (Dilution of 1:1000)
It did not work for HEK293 cell lysates, probably because it does not cross-react with the human LAMP1.
On March 9, 2012 Simon Glerup, Aarhus wrote:
This antibody stains vesicles in 293 cells that appear to be located in the nucleus or just above it.
I doubt that these are lysosomes! 293 cells were permeabilized with 0.1% TritonX100
On February 29, 2012 Anja A, Aarhus wrote:
This antibody also works for staining of 293 cells.
On February 15, 2012 Simon Glerup, Aarhus wrote:
We use this antibody as a lysosomal marker in mouse-derived cell lines, e.g. primary astrocytes and hippocampal neurons.
Dilution 1:300.
NB. For human cell lines, we use mAb H4A3.
It is important to consider what detergent is used for permeabilization of cells. Saponin appears to give much better lysosome stainings than Triton X-100.
For inhibition of lysosomal degradation and better visualization of lysosomes, incubate with 50 ug/ml leupeptin and pepstatin for 24 h before fixing the cells. During this period, the leupeptin/pepstatin medium has to be changed every six hours.