I have tested both 510 and 511 in Western blot. No signal at all, even when used in 1:10 dilution.
Samples: Mouse adult brain WT and SorCS1-/-, p0 WT brain and CHO cells stable expressing either mouse or human SorCS1 variant c.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On October 30, 2012Jenny BF wrote:
4
Animal 511:
This antibody was used to stain CHO cells stable expressing mouse SorCS1 variant c. It stains the membrane specifically,but background staining is also seen in untransfected control cells at 1:100 concentration. The signal/background ratio is best in the second bleed.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 13, 2012Simon Glerup wrote:
4.5
We had this antibody custom made by Genovac using DNA immunization of rabbits with a plasmid encoding mouse SorCS1.
Second bleed is excellent in specifically detecting mouse SorCS1 in Western blotting. No cross reactivity with human SorCS1.
It also stains primary cultures derived from wild-type mice but not from SorCS1 knockouts.
On December 10, 2014 Peter Lund Ovesen wrote:
I have tested both 510 and 511 in Western blot. No signal at all, even when used in 1:10 dilution.
Samples: Mouse adult brain WT and SorCS1-/-, p0 WT brain and CHO cells stable expressing either mouse or human SorCS1 variant c.
On October 30, 2012 Jenny BF wrote:
Animal 511:
This antibody was used to stain CHO cells stable expressing mouse SorCS1 variant c. It stains the membrane specifically,but background staining is also seen in untransfected control cells at 1:100 concentration. The signal/background ratio is best in the second bleed.
On February 13, 2012 Simon Glerup wrote:
We had this antibody custom made by Genovac using DNA immunization of rabbits with a plasmid encoding mouse SorCS1.
Second bleed is excellent in specifically detecting mouse SorCS1 in Western blotting. No cross reactivity with human SorCS1.
It also stains primary cultures derived from wild-type mice but not from SorCS1 knockouts.