This antibody works very well for detection of mouse TrkB. Clear bands of both full-length and splice variant T1 are visible using 1:1000 dilution.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 8, 2017Anders Dalby wrote:
5
Works very well for ICC of primary hippocampal neurons.
Neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with primary antibody in a 1:500 dilution in blocking buffer.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On July 13, 2016Signe Petersen, Aarhus University wrote:
5
Mouse TrkB antibody, AF 1494, R&D Systems
I have used this antibody for western blotting of call lysates from a primary hippocamal neuron culture in a dilution of 1:1000. This antibody works so well for me, that I often use it as a control of my blotting protocol.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On May 2, 2015Lars Meinertz Byg, Aarhus University wrote:
5
Also works extremely well with immunocytochemistry of human TrkB. i aplied it in a 2 ug/ml and got a clearcut window with no control-staining
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On April 15, 2015Lars Meinertz Byg, Aarhus University wrote:
5
This antibody works great for IP and westernbloting at dilutions:
WB 1/1000
IP 1/1000
Western blot
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On March 13, 2015Simon Mølgaard, Aarhus University wrote:
5
I have used this antibody for IHC on hippocampaltissue from 10 days old mice. A clear staining was observed which was not seen in the negative control.
Concentration: 1:500
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On January 19, 2015Hande Login, Aarhus University wrote:
4.5
I have used this antibody for western blot and IHC on mouse olfactory bulb and mouse hippocampus. This antibody is extremely good for western blot. But IHC staining for hippocampus did not work. Also I performed IHC on spinal cord cryosections. It works very good with the right protocol. I recommend to follow the exact protocol for IHC in R&D website.
For western, the dilution is: 1/1000
For IHC on spinal cord cryosections, the dilution is: 1/500
Western blot
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 21, 2014Simon Mølgaard, Aarhus University wrote:
1
I have tested this antibody for IP of human TrkB. Although it works very well for IP of mouse TrkB, it does not work for IP of human TrkB.
Western blot
Not Rated
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On January 29, 2014Simon Mølgaard, Aarhus University wrote:
5
I have tested this antibody for IP as well as western blot on cultured hippocampal neurons with very good results. Similar to Simon Glerups review i detected two bands corresponding to full lenght and splice variant.
IP and WB i used 0.2 ug/ml
Western blot
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On December 21, 2011Simon Glerup, Aarhus University wrote:
5
This antibody is excellent!
We went through 15 different commercial antibodies before finding it.
It works for IP of endogenous TrkB from hippocampal neurons, for ICC and IHC on neurons and cryosections, and for Western blotting. With this antibody you will achieve superior sensitivity when doing Western blotting of tissues and blots can be stripped and reprobed several times and still give a nice clean specific band.
The Western blot above shows full length TrkB and the splice variant TrkB T1 in synaptosomal preparations of mouse hippocampus.
On November 28, 2017 Anders Dalby wrote:
This antibody works very well for detection of mouse TrkB. Clear bands of both full-length and splice variant T1 are visible using 1:1000 dilution.
On November 8, 2017 Anders Dalby wrote:
Works very well for ICC of primary hippocampal neurons.
Neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with primary antibody in a 1:500 dilution in blocking buffer.
On July 13, 2016 Signe Petersen, Aarhus University wrote:
Mouse TrkB antibody, AF 1494, R&D Systems
I have used this antibody for western blotting of call lysates from a primary hippocamal neuron culture in a dilution of 1:1000. This antibody works so well for me, that I often use it as a control of my blotting protocol.
On May 2, 2015 Lars Meinertz Byg, Aarhus University wrote:
Also works extremely well with immunocytochemistry of human TrkB. i aplied it in a 2 ug/ml and got a clearcut window with no control-staining
On April 15, 2015 Lars Meinertz Byg, Aarhus University wrote:
This antibody works great for IP and westernbloting at dilutions:
WB 1/1000
IP 1/1000
On March 13, 2015 Simon Mølgaard, Aarhus University wrote:
I have used this antibody for IHC on hippocampaltissue from 10 days old mice. A clear staining was observed which was not seen in the negative control.
Concentration: 1:500
On January 19, 2015 Hande Login, Aarhus University wrote:
I have used this antibody for western blot and IHC on mouse olfactory bulb and mouse hippocampus. This antibody is extremely good for western blot. But IHC staining for hippocampus did not work. Also I performed IHC on spinal cord cryosections. It works very good with the right protocol. I recommend to follow the exact protocol for IHC in R&D website.
For western, the dilution is: 1/1000
For IHC on spinal cord cryosections, the dilution is: 1/500
On February 21, 2014 Simon Mølgaard, Aarhus University wrote:
I have tested this antibody for IP of human TrkB. Although it works very well for IP of mouse TrkB, it does not work for IP of human TrkB.
On January 29, 2014 Simon Mølgaard, Aarhus University wrote:
I have tested this antibody for IP as well as western blot on cultured hippocampal neurons with very good results. Similar to Simon Glerups review i detected two bands corresponding to full lenght and splice variant.
IP and WB i used 0.2 ug/ml
On December 21, 2011 Simon Glerup, Aarhus University wrote:
This antibody is excellent!
We went through 15 different commercial antibodies before finding it.
It works for IP of endogenous TrkB from hippocampal neurons, for ICC and IHC on neurons and cryosections, and for Western blotting. With this antibody you will achieve superior sensitivity when doing Western blotting of tissues and blots can be stripped and reprobed several times and still give a nice clean specific band.
The Western blot above shows full length TrkB and the splice variant TrkB T1 in synaptosomal preparations of mouse hippocampus.
For WB we use 0.2 ug/ml.
For ICC we use 2 ug/ml