We have been using 9E10 anti-myc for detection of myc-tagged proteins in ELISA and Western blot with no issues. Our 9E10 anti-myc antibody was produced in our core facility.
We use 0.5 ug/mL for both ELISA and WB.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Flow cytometry
Not Rated
Luminex
Not Rated
On November 28, 2017Anders Dalby wrote:
5
This antibody works very well in immunostaining myc-tagged proteins in transfected HEK293 cells.
Concentration: 1ug/mL
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On January 5, 2017Seandean Harwood, Aarhus University wrote:
5
9E10 anti-myc
Widely available, in my case was produced internally
Can be found, for example, at: Thermo Fisher, catalogue number 13-2500
This is the standard antibody used for detecting the c-myc tag at my laboratory. It performs well in the following assays:
ELISA: used at 0.5 ug/mL in 2% BSA PBS, followed by detection with a GAM-HRP
Western blot: used at 0.5 ug/mL in 2% milk powder – PBS, then detected with GAM-HRP
FACS: used at 5 ug/mL in 1% FBS PBS, then detected with GAM-PE.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Flow cytometry
Luminex
Not Rated
On February 11, 2015Amir Tayaranian Marvian, DANDRITE wrote:
5
Very good band in western blot 1:1000 after 1h
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On December 16, 2014Kasper Mølgaard Jensen, Aarhus University wrote:
5
Very good antibody for detecting antibody binding in ELISA.
Used in concentration of 0.5ug/mL
Western blot
Not Rated
Immunostaining
Not Rated
IP
Not Rated
ELISA
Flow cytometry
Not Rated
Luminex
Not Rated
On December 30, 2011Kathrin Weyer, Aarhus wrote:
5
I agree! Very good for IP and Western. Never tried it for IF, though.
Of note, it does not work as capturing antibody in ELISA (although it can be used for detection).
Western blot
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On December 30, 2011Simon Glerup, Aarhus University wrote:
5
Really nice antibody when you are working with myc-tagged proteins.
It works in all three categories.
On July 31, 2018 Simon Lykkemark wrote:
We have been using 9E10 anti-myc for detection of myc-tagged proteins in ELISA and Western blot with no issues. Our 9E10 anti-myc antibody was produced in our core facility.
We use 0.5 ug/mL for both ELISA and WB.
On November 28, 2017 Anders Dalby wrote:
This antibody works very well in immunostaining myc-tagged proteins in transfected HEK293 cells.
Concentration: 1ug/mL
On January 5, 2017 Seandean Harwood, Aarhus University wrote:
9E10 anti-myc
Widely available, in my case was produced internally
Can be found, for example, at: Thermo Fisher, catalogue number 13-2500
This is the standard antibody used for detecting the c-myc tag at my laboratory. It performs well in the following assays:
ELISA: used at 0.5 ug/mL in 2% BSA PBS, followed by detection with a GAM-HRP
Western blot: used at 0.5 ug/mL in 2% milk powder – PBS, then detected with GAM-HRP
FACS: used at 5 ug/mL in 1% FBS PBS, then detected with GAM-PE.
On February 11, 2015 Amir Tayaranian Marvian, DANDRITE wrote:
Very good band in western blot 1:1000 after 1h
On December 16, 2014 Kasper Mølgaard Jensen, Aarhus University wrote:
Very good antibody for detecting antibody binding in ELISA.
Used in concentration of 0.5ug/mL
On December 30, 2011 Kathrin Weyer, Aarhus wrote:
I agree! Very good for IP and Western. Never tried it for IF, though.
Of note, it does not work as capturing antibody in ELISA (although it can be used for detection).
On December 30, 2011 Simon Glerup, Aarhus University wrote:
Really nice antibody when you are working with myc-tagged proteins.
It works in all three categories.
IF and WB dilutions: 1 ug/ml