7 User Reviews

  • We have been using 9E10 anti-myc for detection of myc-tagged proteins in ELISA and Western blot with no issues. Our 9E10 anti-myc antibody was produced in our core facility.

    We use 0.5 ug/mL for both ELISA and WB.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISA55555
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody works very well in immunostaining myc-tagged proteins in transfected HEK293 cells.

    Concentration: 1ug/mL

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • 9E10 anti-myc
    Widely available, in my case was produced internally
    Can be found, for example, at: Thermo Fisher, catalogue number 13-2500

    This is the standard antibody used for detecting the c-myc tag at my laboratory. It performs well in the following assays:

    ELISA: used at 0.5 ug/mL in 2% BSA PBS, followed by detection with a GAM-HRP

    Western blot: used at 0.5 ug/mL in 2% milk powder – PBS, then detected with GAM-HRP

    FACS: used at 5 ug/mL in 1% FBS PBS, then detected with GAM-PE.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISA55555
    Flow cytometry55555
    LuminexNot Rated
  • Very good band in western blot 1:1000 after 1h

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Very good antibody for detecting antibody binding in ELISA.
    Used in concentration of 0.5ug/mL

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ELISA55555
    Flow cytometryNot Rated
    LuminexNot Rated
  • I agree! Very good for IP and Western. Never tried it for IF, though.

    Of note, it does not work as capturing antibody in ELISA (although it can be used for detection).

    Western blot55555
    ImmunostainingNot Rated
    IP55555
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Really nice antibody when you are working with myc-tagged proteins.
    It works in all three categories.

    IF and WB dilutions: 1 ug/ml

    Western blot55555
    Immunostaining55555
    IP55555
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

Leave a review

Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex