Tyrosine hydroxylase rabbit anti-rat Millipore AB152

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5 User Reviews

  • I can highly recommend this antibody:

    Rabbit-anti-tyrosine hydroxylase (TH; Merck Millipore, formerly Chemicon) is an outstanding primary antibody to reveal fine subcellular details of catecholaminergic neurons. Both immunoperoxidase and immunofluorescence labelling result in extremely low background staining of paraformaldehyde-fixed tissues. The properties of AB152 are exemplified for forebrain sections in rats (Riedel et al 2002, Figs 6 and 8), in mice (Michalski et al 2017, J Alzheimer Dis, Figs 3,4 and 6) and in rhesus monkeys (Brauer et al 2000, Fig 1).

    Michalski et al. 2017 J Alzheimer Dis: https://www.ncbi.nlm.nih.gov/pubmed/28671120
    Riedel et al. 2002: https://www.sciencedirect.com/science/article/pii/S0891061801001429?via%3Dihub
    Brauer et al. 2000: https://www.sciencedirect.com/science/article/pii/S0006899300026536?via%3Dihub

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
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    LuminexNot Rated
  • The antibody was used for Immunofluorescence staining of neurons derived from murine ES cells differentiated in culture to investigate the presents of dopaminergic neurons.
    The cells were grown on coated cover slips, fixed for 30min in 4% PFA and treated for 5 min with 0,005% Triton-X-100. To reduce unspecific binding cells are blocked/ quenched with a 1% BSA in PBS solution.
    The Antibody was diluted 1:500. The secondary antibody was a goat anti rabbit 586 diluted 1:800.
    Weak staining was observed as well as a high background. The same results were observed in double staining experiments with Sigma # T5076 mouse-anti-β-III-tub (1:8000).

    Western blotNot Rated
    Immunostaining22222
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  • Used for immunefluorescence on cryosections (10µm) of mouse embryos.
    Stains very well, neurons in VTA and locus coeruleus, fibers in forebrain, sympathetic trunk neurons and adrenal medullary cells with very little background.
    Dilution used was 1:250

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Used for immunohistochemistry on free floating PFA fixed mouse brain sections.
    Works very nicely in mice to detect dopaminergic neurons.
    Dilution 1:750

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
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  • Excellent TH antibody for immunohistochemistry. We even have colleagues saing that “it works like magic”.

    We used it for staining TH positive fibers in the striatum and neurons in the substantia nigra and VTA in PFA fixed mouse cryosections.
    Dilution 1:2000 and visualization by DAB reaction.

    However, it cannot beat the one from Pel-Freez – it is the best TH-antibody for IHC

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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