Phospho-MARCKS (Ser167/170), rabbit mAb clone D13E4, Cell Signaling, 8722

  • 33333
  • No Ratings
  • No Ratings
  • No Ratings
  • No Ratings
  • No Ratings
  • No Ratings
  • No Ratings

Related topics: , , , , ,

One User Review

  • This antibody works well. This antibody was tested on hippocampal tissue lysates from mouse. Clear bands were observed at 75kDa after a few minutes of exposure. However a series of unspecific bands also appeared both above and below.

    Dilution 1:1000

    Lysis buffer:

    • 20 mM MOPS
    • 2 mM EGTA (to bind calcium);
    • 5 mM EDTA (to bind magnesium and manganese);
    • 50 mM sodium fluoride (to inhibit protein-serine phosphatases);
    • 60 mM β-glycerophosphate, pH 7.2 (to inhibit protein-serine phosphatases);
    • 25 mM sodium pyrophosphate (to inhibit protein-serine phosphatases);
    • 2.5 mM sodium orthovanadate (to inhibit protein-tyrosine phosphatases);
    • 50nM phenylarsine oxide (to inhibit protein-tyrosine phosphatases);
    • 1% Triton X-100
    • 0.05% sodium dodecylsulphate (SDS)
    cOmplete™ Protease Inhibitor and DTT added before use.

    Western blot33333
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

Leave a review

Western blot
Flow cytometry