Phospho-Akt (Ser473) rabbit Cell Signaling 9271

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4 User Reviews

  • This antibody was used for immunoblotting of RIBA buffer cell lysates obtained from tumor cell lines PFSK-1 and D324.
    Dilution: 1:1000
    Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody is used for evaluating activity of the EGF-receptor pathway after stimulation or inhibition of EGFR in lung cancer cell lines. The antibody is very reliable. We have found that dilution in 5 % BSA is optimal.
    Dilution: 1:500

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Phospho-Akt (Ser473) rabbit antibody #9271 from Cell Signaling gives one specific band at 60 kDa.

    I have used the antibody for western blotting with 20 ug WCE of cultured primary mouse hippocampal neurons and with 20 ug WCE of the human neuroblastoma cell line SH-SY5Y in the dilution 1:1000 and incubation at +4C ON.

    The antbody can be reused 2 times.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • rabbit Phospho-Akt (cat no 9271) from Cell Signaling works very nicely in WB. Have detected pAKT levels from both cell-lines (MG87 fibroblasts) as well as from embryonic kidney tissue lysates (E14).

    Used dilution 1:1000, 5%BSA/TBS/Tween, incubates O/N at +4C

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex