3 User Reviews

  • A preferred tool for the demonstration of many (but not all) astrocytes is rabbit-anti-GFAP from Dakocytomation (Glostrup, Denmark, Z0334). This immunoreagent raised against the cow antigen found to be an excellent marker for astrocytes and astrogliosis in human tissues by Halliday et al 1996 Neurosci Lett. Rabbit-anti-GFAP was very useful to reveal active astroglia for instance after traumatic brain injury or stroke in rodents (Härtig et al 2009 – Figs 1-5). Notably, this antibody detects astroglia in various animal species and recently also in tenrecs (Mack et al 2018 Brain Struct Funct 2018 – Figs 1 and 2).

    Halliday et al. 1996 Neurosci Lett; https://www.sciencedirect.com/science/article/abs/pii/0304394096125921?via%3Dihub
    Härtig et al. 2009 Journal of Chemical Neuroanatomy: https://www.sciencedirect.com/science/article/pii/S0891061808001257?via%3Dihub
    Mack et al. 2018 Brain Struct Funct: https://link.springer.com/article/10.1007%2Fs00429-018-1730-1

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Used for Immunofluorescence of transfected RN22 cells treated with 4% PFA in PBS for 5 min. Washed 10 min in PBS. And penetrated with 0.5 % Triton x-100 in PBS for 7 min at 4 C.
    Best dilution was 1:500.
    Good beautifully filament staining.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Great antibody for immunocytochemistry and immunohistochemistry on mouse tissues.

    It beautifully stains formaldehyde-fixed mouse primary astrocytes and astrocytes in cryosections of mouse brain.

    We recommend 1:500 up to 1:5000 dilution of the antibody.

    The image above shows a primary mouse cortical astrocyte stained for GFAP (red) and the receptor SorLA (green, SorLA mAb 20C11).

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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