Related topics: Alphabetical list, Secondary antibodies
See list of additional immunoglobulin antibodies at Linscott’s Directory
On May 31, 2019
Teresa Martín wrote:
Used as a secondary antibody in Western Blot. Incubation time: 1 hour.
Antibody was diluted 1:2000 in TBST + milk (5%).
On October 29, 2017
Heidi Gytz Olesen, McGill University, Canada wrote:
Goat anti-rabbit HRP conjugate, DakoCytomation
Used as secondary antibody for western. The membrane was incubated with the secondary antibody in 5% low fat milk powder in PBS-T for 1 h at RT on a shaker. 4×5 min washing steps were then carried out. To visualize the proteins, ECL+ Plus Western Blotting detection System (GE Healthcare) was used according to the manufacturers recommendations
Review: Worked very well
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