Related topics: Alphabetical list, Apoptosis, Autophagy, Cancer biology
See list of additional Bcl-2 antibodies at Linscott’s Directory
On October 29, 2017
Heidi Gytz Olesen, McGill University, Canada wrote:
Mouse anti-Bcl-2 (clone Bcl-2-100), Sigma B3170
1:5000 for immunoblotting
5-7.5 ug for IP (Protein G kit and Co-IP kit)
For immunoblotting I dilute in 5% low fat milk powder in PBS-T and incubate overnight at 4°C.
Dynabeads kits (Invitrogen) were used for IP. Longer incubation of the antibody in the Ab binding and washing buffer of 1 h was used for optimal binding. Bcl-2 has the same size as the light chain, thus crosslinking must be conducted for co-IP
Review: Worked very well overall as it could easily IP Bcl-2 from overexpressing cells and yield a single band by immunoblotting.
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