Related topics: Alphabetical list, Transcription factors
See list of additional CREB antibodies at Linscott’s Directory
On October 3, 2018
Michelle Pedersen wrote:
I have tested this antibody on mouse HepG2 cells.
50ug protein were loaded in each well for western blot.
The membrane was left to incubate over night at 4 C in blocking buffer (milk).
The antibody did not work.
On September 21, 2018
Anders Dalby wrote:
This antibody works well on mouse liver lysates to detect total CREB however does not work on primary murine neuronal lysates. Dilution 1:1000.
On January 8, 2017
Signe Petersen, Aarhus University wrote:
CREB (48H2), Rabbit mAb #9197, Cell Signaling Technologies
I have used this antibody for detection of total CREB without any valuable results.
Western blotting of murine hippocampal neurons in a dilution of 1:1000, as recommended by the supplier, did not yield any results. Reprobing the same blot with beta-actin proved that the blotting procedure was succesful.
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