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See list of additional PCSK9 antibodies at Linscott’s Directory
On December 13, 2018
Kasper Budolph Pedersen wrote:
Good antibody, used for IF (1:100) for detection of PCSK9 in HEK CCG cell-line. Incubation for 2h at room temperature.
On February 22, 2018
Julie Torvund-Jensen wrote:
Works nicely for IF detection of exogenously PCSK9 added to cells (HepG2/HEK293).
I have used 1:100 dilution and incubation either for 2 hours at room temperature or overnight at 4C.
On March 15, 2016
Sune Skeldal, Aarhus University wrote:
Easily pulls down purified PCSK9 using 0.9 µg/sample (less would probably do).
On March 25, 2015
Laura Tvilling Poulsen (Aarhus University) wrote:
Anti-PCSK9, AF3888, sheep
We used this antibody for IF (1:100) detection of PCSK9 overexpression in HepG2 cells. The antibody only gives weak IF staining of endogenous PCSK9 in HepG2 cells and an intense signal in HepG2 cells transfected with PCSK9.
On May 26, 2013
Camilla Gustafsen wrote:
This antibody works nicely for IF detection of hPCKS9 in transfected HepG2 cells. In this cell line the endogenous level of PCSK9 only gives a weak signal with 2µg/ml antibody
On March 23, 2012
Simon G, Aarhus wrote:
Works nicely for IP of human PCSK9
On December 22, 2011
Simon Glerup, Aarhus wrote:
This antibody works for IF on cells transfected with human PCSK9 cDNA. Very nice staining.
In our hands it doesn´t seem to recognize commercially available PCSK9 recombinant protein (Circulex, R&D Systems).
It works nicely for Western blotting and IP of recombinant human PCSK9 expressed in 293 and HepG2 cells.
So far we have no experienced with endogenous PCSK9 levels.
Western: 0.2 ug/ml
IF: 2 ug/ml
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