4 User Reviews

  • GAD67 MOUSE MAB MILLIPORE MAB5406
    I have used this antibody to do western blot on mouse frozen tissue. I have incubated with this antibody (1:5000) at 4 °C over night, then with secondary antibody at room temperature for 1 hour. The antibody gives nice band with almost no background.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • I’ve used this antibody for DAB and immunofluorescence in fixed mouse brain (both cryosectioned and vibratomed), and it consistently works well, with high signal and low noise. I’ve also used for Western blot analysis in mouse cortex and cerebellum, and the antibody gives one band at the specified molecular weight with no background/non-specific bands.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This was tested on adult mouse brain cryo sections at a concentration of 1:500. This worked very well.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • I have used this antibody for immunofluorescence on adult mouse brain cryosections.
    It works well, with positive cells clearly defined. Also stains many punctae in the layers of hippocampus.
    Concentration 1:500

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex