13 User Reviews

  • This antibody works great to visualize the cytoskeleton of HEK293 cells. Dilution 1:1000.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Very good antibody that gives a strong signal.
    Dilution 1:5000

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody was used as Western blot loading control for lysates of human CNS embryonal tumors, medulloblastomas and tumor cell lines D283, D324 and PFSK-1.
    Dilution: 1:5000 (1x TBST with 5% w/v BSA)

    Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Works very well for loadning control for western blot on lysates from mouse striatum and cortex. Dilution 1:5000.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • A excellent antibody for Western blots. I have diluted this antibody 1:10.000 and still get a very high, specific signal from HepG2 cells. The antibody solution can be reused several times.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • I have used this antibody as af loading control for mouse liver and adrenal gland homogenates (20-60 µg total protein). I dilute 1:5000 and reuse the antibody several times

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody works well in western blotting using 1:3000 dilution.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • I used this as a primary antibody for western blotting of cultured hippocampal neurons at a dilution of 1:5000 with excellent results.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • I routinely use this antibody as a western blotting loading control in a dilution of 1:5000. It gives a very strong band when using 10 ug cell lysate. I have tried it on rat cell cultures and human cell cultures as well as mouse primary neurons.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • I tried this antibody in a 1:1000 dilution in immunoprecipitation of Human b-actin (SY5Y-cells), with poor results. however, it works great for westernblotting in a 1:3000 solution, and can easily be reused.

    Western blot55555
    ImmunostainingNot Rated
    IP11111
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • i have used this for wb loading control from mouse hippocampal tissue lysates. Works excellent for detection.

    used at a dilution of 1:5000. Can be frozen and reused.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • We have used this antibody (1:2500) for WB loading control (HepG2 lysate).

    Western blot44444
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Great antibody in Western blotting. Very high signal. In our Western blotting imaging system, we get a strong distinct within seconds.

    Works also on stripped membranes. The diluted used antibody can also be frozen and used again.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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