Antibodies for target: Secondary antibodies

Peroxidase anti-Mouse IgG (Fc specific), goat pAb, Sigma-Aldrich, A2554

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Goat Anti-Mouse IgG, Human ads-HRP Cat. # 1030-05 from SouthernBiotech

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Goat anti-mouse IgG (Fc specific) HRP conjugate, secondary antibody, Sigma Aldrich, A2554

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goat anti-mouse IgG, R-Phycoerythrin (RPE) conjugated AffiniPure F(ab’)2 Fragment, Jackson ImmunoResearch, 115-116-071

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Goat anti-mouse Alexa 488, Invitrogen cat. A-11001

Fig 2 Kernohan
Kernohan et al. used this antibody in MethodsX 2014. The figure from the paper shows confocal images of dual labelled DNA FISH in fixed mouse brain sections. (a) Example of field of view of neonatal mouse brain. Each cell has two foci per probe marking the two alleles. (b) Example of a collapsed image showing nuclear DNA foci and (c) its corresponding three dimensional rendering conducted with the Volocity software package. This data can be used to measure relative distances, signal volume or number of foci within the cell.

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Goat anti-Mouse IgG (H+L) Horseradish Peroxidase conjugate, Jackson ImmunoResearch, 115-035-166.

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IgG (rabbit), goat pAb, DyLight 594 conjugated, Thermo Scientific, 35560

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Mouse anti-Human Ig (kappa chain), mAb clone SB81a, FITC conjugated, antibodies-online.com, ABIN376214

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Mouse IgG (H+L) IRDye800 conjugate, donkey pAb, Rockland Immunochemicals, 610-732-002

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Anti Human IgG1 (clone MH161-1), mouse mAb, Sanquin Blood Supply, M1325

See list of additional Human IgG1 antibodies at Linscott’s Directory

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