Related topics: Alphabetical list, Neurotransmission
See list of additional Parvalbumin antibodies at Linscott’s Directory
On May 22, 2017
Abdel-Rahman Al-Absi wrote:
PARVALBUMIN RABBIT ANTI-RAT ABCAM AB11427
I have used this antibody to do western blot on mouse frozen tissue. I have incubated with this antibody (1:1000) at 4 °C over night, then with secondary antibody at room temperature for 1 hour. The antibody gives weak band.
On June 30, 2016
Niels Degn, Aarhus University wrote:
I have used this antibody for IHC on crysections from PFA perfusion fixed mouse brains. Works very well for detection of parvalbumin positive interneurons in the striatum in a dilution of 1:500.
On February 18, 2016
Veerle Paternoster, Aarhus University wrote:
Tested on mouse brain cell lyste (loaded 30 ug and 10 ug) using stainfree gel/membrane, semi-dry blotting and detected with ECL signal.
A weaker band, of appropriate size, together with some more intense bands of other sizes.
used dilution 1/1000.
On November 12, 2014
Simon Mølgaard, Aarhus University wrote:
This was tested os hippocampal lysates from p20 mice. It gave a weak staining with some background.
used at 0.1ug/ml
On August 8, 2014
Maj Ulrichsen, Aarhus University wrote:
I have used this antibody on 20 um spinal cord cryo-sections. It was diluted 1:500 and detected with a fluorescent secondary antibody. Intense parvalbumin-positive cells were very easy to distinguish from background staining. They were small cells and parvalbumin-immunoreactive in the soma and the nucleus. Also, what appeared to be neuronal processes stained positive for parvalbumin in some areas of the spinal cord.
On March 28, 2012
Simon Jensen, Aarhus University wrote:
I used this antibody for staining of hippocampal nuerons in culture as well as staining of tissue, both with fluorescence as well as DAB staining. In all cases this antibody works excellent. For DAB staining i used it 1:4000 while it for fluorescent staining of tissue as well as cells in culture was used 1:1000