This antibody works very well for detection of endogenous p75 in 100 ug lysates of primary rat Schwann cells. Dilution: 1:5000.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On December 30, 2011Simon Glerup, Aarhus wrote:
2.7
This antibody works OK for Western blotting with lysates from cells transfected with rat p75 cDNA. And also for immunoprecipitation using such sample.
However, for visualization of endogenous protein by Western blotting, I cannot recommend it.
On May 22, 2015 Maj Ulrichsen wrote:
This antibody works very well for detection of endogenous p75 in 100 ug lysates of primary rat Schwann cells. Dilution: 1:5000.
On December 30, 2011 Simon Glerup, Aarhus wrote:
This antibody works OK for Western blotting with lysates from cells transfected with rat p75 cDNA. And also for immunoprecipitation using such sample.
However, for visualization of endogenous protein by Western blotting, I cannot recommend it.
It does not work for immunofluorescense.
The WB dilution we use: 1:750