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On June 10, 2020
Kasper Mølgaard Jensen wrote:
Amine reactive dye that bind free amines both in the interior of cells and on surface of cells with compromised cell surfaces. Dye is excited at 633 nm or 635 nm so I use APC-Cy7 channel to detect dead cells along with CD3+ cells in a multi color flow cytometry panel to stain PBMCs and expanded T cells. It works really well in both phenotyping and intracellular cytokine staining (CD Ab from BD Horizon #563800).
Live/dead dye is diluted 1:80 in PBS supplemented with 2 % FBS and cells are then stained with 10 uL of the diluted Live/dead sulution for 20 minutes.