On July 6, 2020Jamie Rose, University of Edinburgh wrote:
4
Free floating sections – IHC and IF require 5% milk blocking solution and 5% citric acid antigen retrieval at 1:1000. For paraffin staining 1:1000 with 2% EDTA and 5% citric acid antigen retrieval.
We tried this Iba1 antibody on PFA-fixed brain sections from mouse.
It gave very faint staining of something that might look like microglia.
I cannot recommend this antibody.
On July 6, 2020 Jamie Rose, University of Edinburgh wrote:
Free floating sections – IHC and IF require 5% milk blocking solution and 5% citric acid antigen retrieval at 1:1000. For paraffin staining 1:1000 with 2% EDTA and 5% citric acid antigen retrieval.
IHC: 5 stars
IF: 4 stars
For more information see Pickett et al. Cell Reports 2019, “Amyloid Beta and Tau Cooperate to Cause Reversible Behavioral and Transcriptional Deficits in a Model of Alzheimer’s Disease”.
https://www.sciencedirect.com/science/article/pii/S2211124719315268?dgcid=rss_sd_all#!
On May 20, 2012 Simon G, Aarhus wrote:
We tried this Iba1 antibody on PFA-fixed brain sections from mouse.
It gave very faint staining of something that might look like microglia.
I cannot recommend this antibody.