I have used this antibody for immunocytochemistry of primary hippocampal neurons in a dilution of 1:500. It worked great.
This antibody worked great for western blotting as well.
Western blot
Immunostaining
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On December 4, 2019Takahito Higashi, The University of Tokyo wrote:
5
This antibody works with immunostaining for labeling recombinant HA-tagged protein in a primary hippocampal neuron in a dillution 1:1000. The antibody works great for western blotting as well.
For more information see Higashi et al., Synapse Elimination Triggered by BMP4 Exocytosis and Presynaptic BMP Receptor Activation, Cell Reports, vol 22, issue 4, p919-929, January 23, 2018 (https://doi.org/10.1016/j.celrep.2017.12.101).
On December 20, 2019 Yutaro Kashiwagi, The University of Tokyo wrote:
I have used this antibody for immunocytochemistry of primary hippocampal neurons in a dilution of 1:500. It worked great.
This antibody worked great for western blotting as well.
On December 4, 2019 Takahito Higashi, The University of Tokyo wrote:
This antibody works with immunostaining for labeling recombinant HA-tagged protein in a primary hippocampal neuron in a dillution 1:1000. The antibody works great for western blotting as well.
For more information see Higashi et al., Synapse Elimination Triggered by BMP4 Exocytosis and Presynaptic BMP Receptor Activation, Cell Reports, vol 22, issue 4, p919-929, January 23, 2018 (https://doi.org/10.1016/j.celrep.2017.12.101).