Tested with IHC on DRG sections from naïve mice either fixation perfused with 4% PFA or perfused with PBS. At our hands, the antibody was found to work best at 1:500 for IHC and did not work with antigen retrieval (microwave in citrate buffer). Staining appear specific toward larger diameter neurons and no nuclear staining was observed.
On September 24, 2019 Ole Ahlgreen wrote:
Tested with IHC on DRG sections from naïve mice either fixation perfused with 4% PFA or perfused with PBS. At our hands, the antibody was found to work best at 1:500 for IHC and did not work with antigen retrieval (microwave in citrate buffer). Staining appear specific toward larger diameter neurons and no nuclear staining was observed.