This antibody was used for detection of recombinant HSPB7 by WB and IP. Works fine for WB, but for IP with Protein G-sepharose binding capacity seems to be not very high. No WB signal was observed from endogenous protein in cell lysate.
For more information see Mymrikov et al., The Chaperone Activity and Substrate Spectrum of Human Small Heat Shock Proteins, The Journal of Biological Chemistry, November 30, 2016 (http://www.jbc.org/content/292/2/672.long).
On September 2, 2019 Evgeny Mymrikov, Institute of Biochemistry and Molecular Biology, University of Freiburg wrote:
This antibody was used for detection of recombinant HSPB7 by WB and IP. Works fine for WB, but for IP with Protein G-sepharose binding capacity seems to be not very high. No WB signal was observed from endogenous protein in cell lysate.
For more information see Mymrikov et al., The Chaperone Activity and Substrate Spectrum of Human Small Heat Shock Proteins, The Journal of Biological Chemistry, November 30, 2016 (http://www.jbc.org/content/292/2/672.long).