This antibody works very well in western blotting and immunoprecipitation. The antibody was used to detect recombinant HSPB4 protein by WB. No signal was observed from endogenous protein of cell lysates. IP was done with recombinant protein.
For more information see Mymrikov et al., The Chaperone Activity and Substrate Spectrum of Human Small Heat Shock Proteins, The Journal of Biological Chemistry, November 30, 2016 (http://www.jbc.org/content/292/2/672.long).
On September 2, 2019 Evgeny Mymrikov, Institute of Biochemistry and Molecular Biology, University of Freiburg wrote:
This antibody works very well in western blotting and immunoprecipitation. The antibody was used to detect recombinant HSPB4 protein by WB. No signal was observed from endogenous protein of cell lysates. IP was done with recombinant protein.
For more information see Mymrikov et al., The Chaperone Activity and Substrate Spectrum of Human Small Heat Shock Proteins, The Journal of Biological Chemistry, November 30, 2016 (http://www.jbc.org/content/292/2/672.long).