Alpha Synuclein (pSer129), rabbit pAb, StressMarq, SPC-742

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One User Review

  • This antibody is very good for fluorescence IHC staining of α synuclein on free floating brain sections.

    Mice were injected with α-synuclein AAV vector in the right hemisphere (striatum). The left hemisphere was left untreated. After 4 months the mice were sacrificed. Their brains were removed and fixed in PFA and cut into 20 µm sections. The tissue was stained using a free floating immunohistochemistry protocol. Clear streaks of stained α-synuclein were visible at the site of injection compared to control.

    Sections were collected in a multi-well plate with 300 µl 1x target retrieval solution (Dako, #S1699), incubated at 70 °C for 30 minutes and allowed cool at room temperature.
    The sections were rinsed for 2 minutes in water, 3×10 minutes in TBS and 30 minutes in 1%BSA/0.3% Triton X/TBS and allowed to incubate with the 1:500 α synuclein antibody in TB buffer (50 mM) overnight at 4 °C with gentle agitation.
    The staining was left at room temperature for 1 hour and subsequently rinsed 3×10 minutes in TBS. Sections were incubated with 1:300 secondary antibody in TB (50 nM) for 4 hours at room temperature. The sections were rinsed 3×10 minutes in TBS with hoechst added the last 10 minutes.
    Sections were mounted on slides and allowed to dry.

    Pictures were obtained using confocal microscopy.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM