LC3B, rabbit pAb, Novus Biologicals, NB100-2220

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2 User Reviews

  • This antibody works great for detection of LC3-I and LC3-II in primary neurons from mice (cortical). A clear signal was obtained after short exposure.
    Dilution 1:1000

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • This antibody works very well for WB detection of LC3-I and LC3-II. Dilution of 1:1000.

    I was also able to do ICC of primary hippocampal neurons with this autophagosome marker, dilution 1:200. (4% PFA for fixation and 0.1% triton for permeabilization.)

    Western blot55555
    Immunostaining44444
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM